vitro antiproliferative assay normal human dermal fibroblast nhdf cell lines  (PromoCell)

Journal: Pharmaceutics

Article Title: Transfersome-Based Delivery of Optimized Black Tea Extract for the Prevention of UVB-Induced Skin Damage

doi: 10.3390/pharmaceutics17080952

Figure Lengend Snippet: Cell viability, evaluated by MTT assay, of Normal Human Dermal Fibroblasts (NHDF) treated for 24 h with different concentrations (1–200 μg/mL) of black tea extract in solution (BT) or in transfersomes (BTT) and empty transfersomes (ET). Data are expressed as the means ± SD of three independent experiments ( n = 3) and were analysed by one-way ANOVA followed by Tukey’s post-hoc test. ** p < 0.01, *** p < 0.001, **** p < 0.0001, vs . CTRL (100% of viability).

Article Snippet: The Normal Human Dermal Fibroblast cell line (NHDF; Cat. no. C-12302) and fibroblast growth medium 2 (Cat. no. C-23020) were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: MTT Assay

Journal: Pharmaceutics

Article Title: Transfersome-Based Delivery of Optimized Black Tea Extract for the Prevention of UVB-Induced Skin Damage

doi: 10.3390/pharmaceutics17080952

Figure Lengend Snippet: Cell viability, evaluated by MTT assay of Normal Human Dermal Fibroblasts (NHDF) treated for 24 h with different concentrations (1–200 μg/mL) of black tea extract in solution (BT) or in transfersomes (BTT), then irradiated with UVB (100 mJ/cm 2 ). Data are expressed as the means ± SD of three independent experiments ( n = 3) and were analysed by one-way ANOVA followed by Tukey’s post-hoc test. **** p < 0.0001, *** p < 0.001, * p < 0.05 vs . UVB+, #### p < 0.0001 vs . CTRL (100% of viability).

Article Snippet: The Normal Human Dermal Fibroblast cell line (NHDF; Cat. no. C-12302) and fibroblast growth medium 2 (Cat. no. C-23020) were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: MTT Assay, Irradiation

Journal: Pharmaceutics

Article Title: Transfersome-Based Delivery of Optimized Black Tea Extract for the Prevention of UVB-Induced Skin Damage

doi: 10.3390/pharmaceutics17080952

Figure Lengend Snippet: Collagen production was evaluated using Sirius red staining of Normal Human Dermal Fibroblasts (NHDF) treated for 24 h with different concentrations (1–200 μg/mL) of black tea extract in solution (BT) or in transfersomes (BTT), then irradiated with UVB (100 mJ/cm 2 ). Data are expressed as the mean ± SD of three independent experiments ( n = 3) and were analysed using one-way ANOVA, followed by Tukey’s post-hoc test. **** p < 0.0001, ** p < 0.01, * p < 0.05 vs . UVB+, #### p < 0.0001 vs . CTRL (100% collagen production).

Article Snippet: The Normal Human Dermal Fibroblast cell line (NHDF; Cat. no. C-12302) and fibroblast growth medium 2 (Cat. no. C-23020) were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Staining, Irradiation

Journal: Pharmaceutics

Article Title: Transfersome-Based Delivery of Optimized Black Tea Extract for the Prevention of UVB-Induced Skin Damage

doi: 10.3390/pharmaceutics17080952

Figure Lengend Snippet: Normal Human Dermal Fibroblasts (NHDF) were treated for 24 h two concentrations (10 and 100 μg/mL) of black tea extract in solution (BT) or in transfersomes (BTT), then irradiated with UVB (100 mJ/cm 2 ). Gene expression of MMP1 ( A ), IL6 ( B ), COX2 ( C ), and iNOS ( D ) was analysed by real time PCR normalized with the housekeeping gene β -actin. Data are expressed as the means ± SD of three independent experiments ( n = 3) and were analysed by one-way ANOVA followed by Tukey’s post-hoc test. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

Article Snippet: The Normal Human Dermal Fibroblast cell line (NHDF; Cat. no. C-12302) and fibroblast growth medium 2 (Cat. no. C-23020) were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Irradiation, Gene Expression, Real-time Polymerase Chain Reaction